ABSTRACT
Objective To investigate the molecular characteristics of dengue virus serotype 2 (DENV2) in Guangzhou during 2001-2015,and analyze the E gene of the strains isolated,the phylogenetic tree and molecular clock were constructed to know about the evolution of the strains.Methods The serum samples of the patients were detected by real time PCR,and positive samples were used to isolate dengue virus by using C6/36 cells.The E gene of the isolated strains were sequenced.The phylogenetic tree was constructed by using software Mega 4.0,and the molecular clock was drawn by using software BEASTv1.8.2.Results Twenty-six dengue virus strains were isolated between 2001 and 2015.They were all clustered into 2 genotypes,i.e.cosmopolitan genotype and Asian genotype Ⅰ.The strains isolated in Guangzhou shared high homology with Southeast Asian strains.The cosmopolitan genotype was divided into 2 sub-genotype at about 46 and 35 years ago.The substitution rate of dengue virus serotype 2 in Guangzhou was 7.1 × 10-4 per year per site.Conclusions There were close relationship between the Guangzhou strains and Southeast Asian strains.Guangzhou was at high risk of imported dengue fever,outbreak of dengue hemorrhagic fever and dengue shock syndrome.There might be two ways of introduction of cosmopolitan genotype.The substitution rate of the strains in Guangzhou was similar to that in the neighbor countries.
ABSTRACT
Objective To investigate the molecular characteristics of dengue virus serotype 2 (DENV2) in Guangzhou during 2001-2015,and analyze the E gene of the strains isolated,the phylogenetic tree and molecular clock were constructed to know about the evolution of the strains.Methods The serum samples of the patients were detected by real time PCR,and positive samples were used to isolate dengue virus by using C6/36 cells.The E gene of the isolated strains were sequenced.The phylogenetic tree was constructed by using software Mega 4.0,and the molecular clock was drawn by using software BEASTv1.8.2.Results Twenty-six dengue virus strains were isolated between 2001 and 2015.They were all clustered into 2 genotypes,i.e.cosmopolitan genotype and Asian genotype Ⅰ.The strains isolated in Guangzhou shared high homology with Southeast Asian strains.The cosmopolitan genotype was divided into 2 sub-genotype at about 46 and 35 years ago.The substitution rate of dengue virus serotype 2 in Guangzhou was 7.1 × 10-4 per year per site.Conclusions There were close relationship between the Guangzhou strains and Southeast Asian strains.Guangzhou was at high risk of imported dengue fever,outbreak of dengue hemorrhagic fever and dengue shock syndrome.There might be two ways of introduction of cosmopolitan genotype.The substitution rate of the strains in Guangzhou was similar to that in the neighbor countries.